Multiple-Locus Variable number tandem repeat Analysis (MLVA) is a method used to perform molecular typing of particular microorganisms. It utilizes the naturally occurring variation in the number of tandem repeated DNA sequences found in many different loci in the genome of a variety of organisms. The method originates from forensic science where it is used for DNA fingerprinting in samples from human origin. MLVA is also widely used to assess the molecular fingerprint of micro-organisms such as bacteria. The molecular typing profiles are use to study transmission routes, to assess sources of infection and also to assess the impact of human intervention such as vaccination and use of antibiotics on the composition of bacterial populations.