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Multiple alignment
A computational method that lines up, as a set of rows of text, three or more sequences (of DNA, RNA, or proteins) to maximize the identity of overlapping positions while...-
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Multiple Locus Variable Tandem Repeat Analysis (MLVA)
Multiple-Locus Variable number tandem repeat Analysis (MLVA) is a method used to perform molecular typing of particular microorganisms. It utilizes the naturally occurring...-
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SNP analysis
Detection of Single Nucleotide Polymorphisms in WGS data from a set of indiviual isolates, followed by a cluster analyses based on the SNPs-
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Data
Facts, measurements, recordings, records, or observations about the world collected by scientists and others, with a minimum of contextual interpretation. Data may be in any...-
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SNP tree
Comparison of Single Nucleotide Polymorphisms can be compared across several isolates and the differences can be used to create trees depicting relatedness among the isolates.-
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Sequence alignment
An algorithmic approach to find the best matching of consecutive letters in one sequence (text symbols that represent the polymer subunits of DNA or protein sequences) with...-
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Shared Definition
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Variant detection
NGS is frequently used to identify mutations in DNA samples from individual patients or experimental organisms. Sequencing can be done at the whole-genome scale; RNA-seq, which...-
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Consensus Sequence
When two or more DNA sequences are aligned, the overlapping portions can be combined to create a single consensus sequence. In positions where all overlapping sequences have the...-
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Data Pseudomysation
The processing of personal data in such a way that the data can no longer be attributed to a specific data subject without the use of additional information, as long as such...-
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Sequence fragment
A short string of text that represents a portion of a DNA (or RNA) sequence. NGS machines produce short reads that are sequence fragments that are read from DNA fragments.-
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Sequence variants
Differences at specific positions between two aligned sequences. Variants include single-nucleotide polymorphisms (SNPs), insertions and deletions, copy number variants, and...-
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Continuous Data Collection
Investigator-initiated active collection of animal health related data from individual units in a population which is not limited to a particular time period, to assess the...-
Animal Health
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Animal Health
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Contig
A contiguous stretch of DNA sequence that is the result of assembly of multiple overlapping sequence reads into a single consensus sequence. A contig requires a complete tiling...-
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Sequence read
When DNA sequence is obtained by any experimental method, including both Sanger and next-generation methods, the data are obtained from individual template molecules as a string...-
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Next Generation Sequencing (NGS)
DNA sequencing technologies that simultaneously determine the sequence of DNA bases from many thousands (or millions) of DNA templates in a single biochemical reaction volume....-
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Amplicon
An amplicon is a specific fragment or locus of DNA from a target organism (or organisms), generally 200–1000 bp in length, copied millions of times by the polymerase chain...-
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Cluster Detection
Identifying groups of things that are clustered together according to their similarity to each other by some measure; this includes (but is not limited to) pathogen isolates...-
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Personal Data
Means any information relating to an identified or identifiable natural person (‘data subject’); anidentifiable natural person is one who can be identified, directly or...-
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Library Preparation
Peparing purified DNA for NGS. Fragmentizes long DNA strands and ligates primers and other sequencing related bits.-
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