34 items found

Types: GlossaryTerm Tags: Sampling and laboratory testing Data processing and analysis

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  • GlossaryTerm

    Multiple alignment

    A computational method that lines up, as a set of rows of text, three or more sequences (of DNA, RNA, or proteins) to maximize the identity of overlapping positions while...
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  • GlossaryTerm

    Multiple Locus Variable Tandem Repeat Analysis (MLVA)

    Multiple-Locus Variable number tandem repeat Analysis (MLVA) is a method used to perform molecular typing of particular microorganisms. It utilizes the naturally occurring...
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  • GlossaryTerm

    SNP analysis

    Detection of Single Nucleotide Polymorphisms in WGS data from a set of indiviual isolates, followed by a cluster analyses based on the SNPs
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  • GlossaryTerm

    Data

    Facts, measurements, recordings, records, or observations about the world collected by scientists and others, with a minimum of contextual interpretation. Data may be in any...
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  • GlossaryTerm

    SNP tree

    Comparison of Single Nucleotide Polymorphisms can be compared across several isolates and the differences can be used to create trees depicting relatedness among the isolates.
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  • GlossaryTerm

    Sequence alignment

    An algorithmic approach to find the best matching of consecutive letters in one sequence (text symbols that represent the polymer subunits of DNA or protein sequences) with...
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  • GlossaryTerm

    Sequence Data

    The raw reads produced from basecalling, usually saved in FASTQ format for NGS data.
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  • GlossaryTerm

    Variant detection

    NGS is frequently used to identify mutations in DNA samples from individual patients or experimental organisms. Sequencing can be done at the whole-genome scale; RNA-seq, which...
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  • GlossaryTerm

    Consensus Sequence

    When two or more DNA sequences are aligned, the overlapping portions can be combined to create a single consensus sequence. In positions where all overlapping sequences have the...
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  • GlossaryTerm

    Data Pseudomysation

    The processing of personal data in such a way that the data can no longer be attributed to a specific data subject without the use of additional information, as long as such...
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  • GlossaryTerm

    Sequence fragment

    A short string of text that represents a portion of a DNA (or RNA) sequence. NGS machines produce short reads that are sequence fragments that are read from DNA fragments.
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  • GlossaryTerm

    Sequence variants

    Differences at specific positions between two aligned sequences. Variants include single-nucleotide polymorphisms (SNPs), insertions and deletions, copy number variants, and...
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  • GlossaryTerm

    Continuous Data Collection

    Investigator-initiated active collection of animal health related data from individual units in a population which is not limited to a particular time period, to assess the...
    • Animal Health
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  • GlossaryTerm

    Contig

    A contiguous stretch of DNA sequence that is the result of assembly of multiple overlapping sequence reads into a single consensus sequence. A contig requires a complete tiling...
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  • GlossaryTerm

    Sequence read

    When DNA sequence is obtained by any experimental method, including both Sanger and next-generation methods, the data are obtained from individual template molecules as a string...
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  • GlossaryTerm

    Next Generation Sequencing (NGS)

    DNA sequencing technologies that simultaneously determine the sequence of DNA bases from many thousands (or millions) of DNA templates in a single biochemical reaction volume....
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  • GlossaryTerm

    Amplicon

    An amplicon is a specific fragment or locus of DNA from a target organism (or organisms), generally 200–1000 bp in length, copied millions of times by the polymerase chain...
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  • GlossaryTerm

    Cluster Detection

    Identifying groups of things that are clustered together according to their similarity to each other by some measure; this includes (but is not limited to) pathogen isolates...
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  • GlossaryTerm

    Personal Data

    Means any information relating to an identified or identifiable natural person (‘data subject’); anidentifiable natural person is one who can be identified, directly or...
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  • GlossaryTerm

    Library Preparation

    Peparing purified DNA for NGS. Fragmentizes long DNA strands and ligates primers and other sequencing related bits.
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